UJI AKTIVITAS PESTISIDA NABATI SECARA IN VITRO
ABSTRAK: Biopesticide
materials extracted from union, mimba leafs, peper betle leafs, lemon grass,
and comercial bactericide as control. Bacteria soruces by isolation from rotten
cabbage and pineapple. Medium composition of nutrien agar are vitabro 1 g/L,
vitamineral 4 g/L, sucrose 7 g/L, skim milk 2 g/L, and agar 11 g/L, whereas
broth composition is not added agar. Pure microbial was made by strep method.
Inhibition zone of antimicrobe was measured by formula in mm2. Isolation result
from rotten cabbage are four coccus bacteria and one bacil bacteria with four
positives and one negative gram bacteria. Isolate bacteria from pineapple were
one negative and one positive gram. Union extraction had antimicrobe activitiy
highest (average 1200 mm2) than others biopesticide. Union extraction had
inhibits of growth to all bacteria, bacil, coccus, positive and negative gram.
Meanushile peper betle leafs only had antimicrobe activity for coccus negative
gram. Boiling on biopesticide sources at 1000C for 45 second could decreasing
of antimicrobe activity but fermentation process increased of antimicrobeial
activity. Fermentation was increasing of inhibition zone peper betle leafs at
8.6 mm2 on bacil positive gram bacteria, 8.6 mm2 on bacil positive gram, and
30.6 mm2 on coccus negative gram bacteria. Decrease of union activity was
estimated by dilution at fermentation.
Keyword: activity,
biopesticide, microbe, in vitro
Penulis: Mokhamad Irfan
Kode Jurnal: jppertaniandd100077
