Purifikasi Imunoglobulin Yolk Pada Ayam yang Divaksin terhadap Ekskretori/Sekretori Stadium L3 Ascaridia galli
ABSTRACT: The main
immunoglobulin fraction of poultry is called IgY, in order to distinguish it
from the mammalian IgG. This article focus on purification yolk immunoglobulin
of hens vaccinated against excretory/secretory Ascaridia galli larvae to
obtained purity IgY. Active vaccinations with excretory/secretory antigen were
applied intra muscularly of chickens with an initial dose of 80 μg. The
vaccinations were repeated three times with dose of each 60 μg with an interval
of one week. The first vaccinations were excretory/secretory antigen mixed with
Fruend Adjuvant Complete and subsequently mixed with Freund Adjuvant
Incomplete. Antibody response in yolk was detected at weekly intervals by agar
gel precipitation test (AGPT). The chicken’s eggs were collected from 49 day
after vaccinations. IgY was extracted from egg yolks by means of ammonium
sulphate and purified using fast performance liquid chromatography (FPLC). The
purity of anti-ekscretory/secretory IgY protein was determined by Bradford
method (λ = 280 nm). The result showed that antibody in yolk was begun detect
with AGPT at four weeks after vaccination. IgY concentration after purification
was 0,875 ± 0.251 mg/ml. This study has shown that the product released in
vitro by L3 stage A. galli is capable of stimulating humoral immunity by mean
of producing antibody through yolk as biologic manufacturer could be a good
choice.
Keywords: ascaridia galli;
excretory; secretory antigen; yolk Immunoglobulin
Penulis: Darmawi, Ummu Balqis,
Risa Tiuria, Muhammad Hambal, Samadi
Kode Jurnal: jppeternakandd100076
