PENGGUNAAN VITAMIN E DAN BHT DALAM PENGENCER SEMEN BEKU DOMBA
ABSTRAK: A study about the use
ofvitamin E and BHT in frozen ram senten extender has done. Tris citrate,
lactose and skim milk as extenders and vitamin Eand BHT as antioxidants with
ceitain doses were used in this experiment. The quality ofsemen such as
motility, live sperm, intact plasma membrane and intact acrosom membrane were
evaluated. The objectives ofthis study are to determine the best extender and
antioxidant with certain doses in cryopreservation of ram semen. Based on"
this study it was found that the skim milk extender (50.0 and 62.5%
respectively) showed the best quality ofsperm motility and live sperm after
thawing. The mean percentage ofintact plasma membrane and intact acrosome on
skim milk extender also showed the best result (58.3 and 61.0% respectiDely).
The supplement of0.2 g vitamin Eshowed higher rate ofsperm motility, live
sperm, intact plasma membrane and intact acrosome after thawing than the other
treatments (51.9, 65.4, 61.3 and 64.2 percent respectiDely). It can be
concluded that the supplementation of 0.2 g vitamin E using the skim milk
extender gave the best quality in ram semen cryapreservation.
Keyword: antioxidant, BHT,
cryopreseroation, semen, vitamin E
Penulis: FERADIS
Kode Jurnal: jppeternakandd100002
