Penggunaan Gen GH sebagai Marka Molekuler DNA Gurami, Osphronemus goramy dalam Pengembangan Teknologi Surrogate Broodstock
Abstract: The technology of
fish germ cell transplantation had been established to create broodstock
systems by which a target offspring can be produced from a surrogate parent.
Donor cell for transplantation is derived from transgenic fish carrying green
fluorescent protein gene functions as a marker to distinguish the donor from
recipient cell. In this study, an alternative technique was developed for
identifying gouramy-derived donor cell and Nile tilapia as recipient by PCR
amplification method using growth hormone (GH) gene as a molecular marker. Specific primer for GH gouramy was designed
by using Genetyx version 7 software.
ß-actin gene was used as an internal control of DNA loading. The result
showed that a specific PCR amplification product of 340 in length was obtained
when the DNA template was from gouramy, while no PCR product from Nile tilapia.
The minimum concentration of genomic DNA of gouramy mixed with a 700 ng/µl of
Nile tilapia that could be detected by PCR was 1 ng/µl. Thus, PCR method with
specific GH primer may be useful to detect the incorporation of donor cell in
recipient gonad towards development of surrogate broodstock technology.
Keywords: Giant gouramy, GH,
molecular marker, PCR, transplantation
Penulis: Marlina Achmad, Alimuddin,
Odang Carman, Harton Arfah, Muhammad Zairin
Kode Jurnal: jpperikanandd090006
