PENGARUH NISBAH TOTAL ETANOL – PKO DAN WAKTU REAKSI TERHADAP RENDEMEN DAN AKTIVITAS ANTIBAKTERI PRODUK ETANOLISIS MINYAK INTI SAWIT (PKO)
ABSTRACT: he purposes of this
work were to find the effects of ethanol-PKO ratio and reaction time on the
yield and antibacterial activities of palm kernel oil ethanolysis
products. The treatments were ratios of
ethanolyisis mediums and duration of agitation. The medium treatments were ethanol 96% (NaOH 1%; w/w of oil) solution
and PKO at ratio of 0,4; 0,7; 1,0; 1,3 and 1,6 (v/w) and the duration of
agitation on a shaker at 300 rpm for 4, 6, 8, and 10 minutes. Antibacterial activities were tested using
Staphylococcus aureus and Escherichia coli. The results showed ethanol-PKO
ratio at 1,6 (v/w) effectively increased the yield of ethanolysis products for
4 up to 10 min, while the treatments at ratio of 0,4; 0,7; 1,0 (v/w) did not
affect significantly, and at ratio of 1,3 (v/w) was optimum for 4 min. Ethanolysis
products and their fractions for all treatments had antibacterial activities
toward two bacteria (E. coli and S. aureus) with the average diameter of zone
inhibition was 5,38 mm/15 mg. The
optimum ethanol-PKO ratio and time of reaction were 1,3 (v/w) and 10 minutes to
produce ethanolysis products, primarily for fraction 1 (uper layer after
fractionation).
Keywords: Antibacterial activities, ethanolysis, Palm
Kernel Oil
Penulis: Mairisa Lestari,
Murhadi
Kode Jurnal: jppertaniandd080032
