PENGARUH KONSENTRASI KITOSAN TERHADAP AKTIVITAS ANTIBAKTERI DENGAN METODE DIFUSI AGAR (SUMUR)
ABSTRACT: The aim of this work
was to obtain the minimal concentration of chitosan which possess antibacterial
characteristic. The concentrations of chitosan that used were 0%; 0,2%; 0,4%;
0,6%; and 0,8% (w/v), whereas bacteria cultures used to examine antibacterial
activity of chitosan were Escherichia coli, Bacillus subtilis and
Staphylococcus aureus. Well method (gel diffusion) was utilized to test the
chitosan antibacterial activity. The
result was analyzed using descriptive method and presented in the graphic
form. The result showed the activity of
chitosan restraint as antibacterial. The
test of chitosan antibacterial activity utilizes gel diffusion (well) method.
The data analyzed descriptively and presented in the graphic form. The result
showed the activity of chitosan restraint as anti bacteria against
Staphylococcus aureus, Bacillus subtilis or Escherichia coli with the highest
restraint diameter was noticed at the addition of 0,2% chitosan, which were
20,27 mm/mg chitosan, 24,50 mm/mg chitosan and 31,53 mg chitosan,
respectively. The lowest restraint
diameter was found at the addition of 0,8% chitosan, which were 6,82 mm/mg
chitosan, 12,14 mm/mg chitosan and 14,23 mm/mg chitosan, respectively.
Keywords: Antibacterial activity, chitosan, gel
diffusion method
Penulis: Fibra Nurainy, Samsul
Rizal, Yudiantoro
Kode Jurnal: jppertaniandd080034
