Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan JenisKelamin Anak Sesuai Harapan
ABSTRACT: The purposes
of this experiment
are to investigate the
separation of X
and Y spermatozoa by measuring the spermatozoa
quality, sex ratio between
X and Y,
capacity of fertility indicated by
conception rate and
sex ratio of
goat boar kids. Samples,
used in this
experiment, are fresh semen
from Boer goat
with high quality consists of 4 group treatments with 6
replications 1) group of spermatozoa
without separation (control), 2)
group of spermatozoa
separated by percoll gradient density
centrifugation 3 levels
(P1), 5 levels (P2)
and swine up
(P3). The observed parameters are
spermatozoa quality, X
and Y spermatozoa ratio,
fertility’s capacity and sex ratio on
the birth. Quality
examination of spermatozoa and identify
X and Y
spermatozoa is based
on the standard method of WHO.
The conception rate was based on the
ratio of pregnant
goat after the
first insemination. Data of
spermatozoa quality and spermatozoa ratio
were analyzed by
using analisis of variance
(ANOVA) and further analysis by LSD if
there were differences
between treatments. The results
of this experiment
showed that spermatozoa quality Boer
goat significantly reduced
(p<0,05) after separation with
percoll gradient density centrifugation and
swim up. Percentage spermatozoa X
after percoll gradient
density centrifugation was significantly
higher (P<0,05) compared to
control and swim
up. Meanwhile, the Y
spermatozoa population was
significantly higher (P<0,05) after
swim up treatment
compared to percoll gradient
density centrifugation and
control. The percentage of
sex ratio (male:
female) after insemination from
percoll gradient density centrifugation produced more female
than male. On the other hand,
insemination from swim
up produced more male
than female. Sex
ratio produced from separation
of percoll gradient density centrifugation, swim up was
difference from control semen (P<0,05).
From this experiment,
it was concluded that
spermatozoa separation by percoll gradient density centrifugation
and swim up can be
used as one
of the methods
to separate X and Y spermatozoa and further can be
applied to get preferred sex animals.
Key words: Spermatozoa, goat
boer, percoll gradient density centrifugation,
swim up, ratio sex
Penulis: Dasrul, M. Aman Yaman
dan Zulfan
Kode Jurnal: jppeternakandd130012
