Karakterisasi Tiga Lokus Mikrosatelit pada Telur dan Larva Tuna Sirip Kuning, Thunnus albacares
Abstract: Characterization of
three microsatellite loci are able to provide information about genetic
variation or polymorphism of yellow fin tuna eggs and larvae produced by
domestication of broodstock. Thirty samples of eggs and larvae were collected
from different spawning day and analyzed. Amplification of those three loci
were carried out with multiflexing technique and electrophoreted ABI Prism 310
Genetic Analyzer sequencer. From those three loci were used (Ttho-1, Ttho-4 and
Ttho-7) showed that locus Ttho-7 had higher genetic variance (0.720-1.212)
compared to others loci. Based on the result, it can be concluded that high
allele frequency as showed at Ttho-7 could be used as marker for parental analysis.
Keywords: Characterization,
microsatellite, yellow fin tuna, genetic diversity
Penulis: Sari B. Moria, Gusti
N. Permana, Jhon H. Hutapea
Kode Jurnal: jpperikanandd090004
