KARAKTERISASI PARSIAL EKSTRAK KASAR ENZIM PROTEASE DARI Bacillus amyloliquefaciens NRRL BNRRL B-14396
ABSTRACT: This research was
aimed to determine the characteristics of crude proteases extracted from
Bacillus amyloliquefaciens NRRL B-14396, namely the stability of enzyme during
storage at low temperature, the optimum pH and temperature, kinetic constants
(Vm and Km) and some inhibitors and activators that influence the enzyme
activity and to evaluate the stability of enzyme during storage at low
temperature.
The results showed that the stability of protease extracted from Bacillus
amyloliquefaciens NRRL B-14396 decreased with time when stored at 4oC. It was
found two classes of protease, i.e. the one with an optimum pH of 7.5,
classified as the neutral protease, and that with the optimum pH of 9.0, known
as alkaline protease. The activity levels of the neutral and alkaline proteases
were 0.475 Unit/ml and 0.410 Unit/ml, respectively. The optimum temperature was
50oC, at which the activity level was of 0.516 Unit/ml. Ca2+ ion functions as a
stabilizer whereas Zn2+ and Fe2+ ions as activators. The addition Mg2+ ion,
SDS, and EDTA inhibited the activity. The respective values of Km and Vm of the
neutral protease were 0.167% and 0.633 Unit/ml, while those values of the
alkaline protease were 0.661% and 0.924 Unit/ml.
Key words: characterization,
protease, Bacillus amyloliquefaciens
Penulis: Novita W., K. Arief,
F. C. Nisa, U. Mardiyatmo
Kode Jurnal: jppertaniandd060016
