Kajian Titer Antibodi Pada Yolk dari Ayam yang Diimunisasi Dengan Antigen Ekskretori/Sekretori Stadium L3 Ascaridia galli
ABSTRACT: The purpose of the
present study was to trigger humoral immunity of chickens egg yolk exposed to
excretory/secretory released in vitro by L3 stage of Ascaridia galli. Amount of
6 head chickens were divided into two groups. First group, the chickens were
not immunized. Second group, the chickens were immunized with excretory/
secretory. Active immunizations with excretory/ secretory antigen were applied
intra muscularly of chickens with an initial dose of 80 μg. The immunizations
were repeated three times with dose of each 60 μg with an interval of one week.
The first immunizations were excretory/secretory antigen mixed with Fruend
Adjuvant Complete and subsequently mixed with Freund Adjuvant Incomplete.
Antibody response in yolk was detected at weekly intervals by enzyme linked
immunosorbant assay (ELISA). The result showed that antibody in yolk was begun
detect with ELISA increased at two weeks after immunization, This study has
shown that the excretory/secretory released in vitro by L3 stage A. galli is
capable of stimulating humoral immunity by mean of producing IgY in yolk.
Keywords: Ascaridia ;
excretory antigen; secretory antigen; antibody
Penulis: Darmawi, Ummu Balqis,
Risa Tiuria, Muhammad Hambal, Samadi
Kode Jurnal: jppeternakandd080044
