KAJIAN LAMA REAKSI TERHADAP RENDEMEN MASSA DAN AKTIVITAS ANTIMIKROBA PRODUK GLISEROLISIS
ABSTRACT: The study was
conducted to determine the effect of glycerolysis reaction time (48, 72, 96,
120 and 144 hours) on yield and antimicrobial activity of glycerolysis products
from palm kernel oil (PKO) using rice bran as biocatalyst. The result showed that fractionation of
glycerolysis products at all reaction time levels were yielding fraction A
(61,8%) that higher than fraction B (21,8%) and fraction C (16,3%). Generally, all fractions (A, B and C) from
all treatments have antimicrobial activity.
Average of inhibition zone diameter (d) values of fraction C for all
reaction time levels against S. aureus, B. subtilis, E. coli, and S.
cerevisiae, i.e. 7,66; 6,81; 1,33; and 9,09 mm/6 mg fraction,
respectively. Antimicrobial activity of
fraction C was higher than fraction A and B, especially against S. aureus, B.
subtilis, and S. cerevisiae at 72 and 96 h. of reaction times. Escherichia coli is more resistant bacteria
to all fractions than another microbes in this study. All fractions from all reaction time levels
be able to reduce of total mold (A. orizae) colony with each average percentage
of 79,6 (fraction A); 81,0 (fraction B); and 78,2% (fraction C).
Keywords: Glycerolysis, PKO,
fraction, antimicrobial activity, resistant
Penulis: Havita Dwi Anggasari,
Murhadi
Kode Jurnal: jppertaniandd090033
