IMUNOGENISITAS VAKSIN INAKTIF WHOLE CELL AEROMONAS SALMONICIDA PADA IKAN MAS (CYPRINUS CARPIO)
ABSTRACT: The aims of this
research was to determine the immunogenicity of inactivated vaccine A.
salmonicida whole cell in common carp and the best mothod in vaccine
administration. Vaccine was produced by inactivated A. salmonicida with adding
1 ppm (v/v) formaldehide and incubated for 24h in room temperature. Ten juvenil
of carp (Vaccine was administrated in each 10 fish by injection
intraperitoneally (107 cfu/fish), orally (107 cfu/fish), immersion (107 cfu/ml
for 30 minute) and control (fish with no vaccination). Booster was conducted 7
days after first vaccination with same dossage and method. Titer antibody was evaluated
in three times i.e. before vaccination, 7th days after first vaccination, and
7th days after booster. Water quality such as dissolved oxygen, pH, and water
temperature was measured as a supported parameters. Results showed that titer
antibodi for all treatment before vaccination was 1/6. However, titer antibody
after vaccination and booster increased to 1/58.67 and 1/85.33 for i.p
injection, 1/42.67 and 1/64 for oral, 1/24 and 42.67 for immerse, respectively.
Whereas, there was no significantly increasing of titer antibody in control
that was 1/9.33 and 1/18.67 for vaccination and bosster, respectively. Vaccine
adminsitration method by injection i.p. was the best method for obtain the best
immunogenicity of vaccine. Water quality parameters along this experiment still
in optimum range for common carp living.
Keywords: Aeromonas
salmonicida, common carp, inactivated vaccine, titer antibody
Penulis: Agus Setyawan, Siti
Hudaidah, Zulfikar Zafeskan Ronapati, Sumino
Kode Jurnal: jpperikanandd120004
