IDENTIFIKASI GROWTH DIFFERENTIATION FACTOR-9 (GDF-9) PADA OOSIT SAPI YANG DIMATURASI SECARA IN VITRO DENGAN METODE ELEKTROFORESIS
ABSTRACT: The aim of this research
was to identification of protein Growth Differentiation Factor-9 (GDF-9)
isolated from oocytes dominant follicles in in vitro maturation. Bovine ovary
obtained from a slaughterhouse was aspired in its ovary dominant follicles. The
oocytes were matured in Tissue Culture Medium 199 (TCM 199) then culture for 22
hours at 38,5 ºC in incubator CO2. Twenty two hours after being culture, the
GDF-9 protein identification were examined using Sodium Dodecyl Sulphonate
Polyacrilamide Gel Electrophoresis (SDS PAGE). Several protein fractions were
obtained from the result of research. Based on calculation of regression
equation resulting from protein marker to determine the molecular weight of
GDF-9 protein. Eight protein fractions were determined, they are : 177 kDa, 137
kDa, 100 kDa, 73 kDa, 51 kDa, 41 kDa, 38 kDa, 27 kDa, 20 kDa. Protein appearing
in the protein band the molecular weight of 51 kDa was identified as GDF-9
protein playing role in maturation process.
Key word: GDF-9, oocyte,
maturation, SDS PAGE
Penulis: Epy M. L. Widjiati,
Zaenal Mustakim, Lianny Nangoi
Kode Jurnal: jppeternakandd080027
