Identifikasi Cacing Polychaeta, Nereis sp. sebagai Vektor White Spot Syndrome Virus (WSSV) di Alam dan Kajian Uji Tantangnya di Laboratorium
Abstract: Polychaete worm is
an important zoobenthos at shrimp culture system, however since there is no any
culture of these worm in Indonesia, then all the worm biomass collected from
their natural habitat. It raised some concern about their safety because
polychaete worm might be get infected and will realistic as a pathway for virus
to attack shrimp broodstock in the hatchery. Fresh polychaete worms (1 g wet)
used as a test organisms were caught from pond area in Semat region, Jepara.
The step of these experiment are identification of WSSV infection at polychaete
worms in wild with PCR analysis, visual, microscope and histology observation.
Followed by challenge test of polychaete worm with WSSV, and WSSV infectivity
study at black tiger shrimp broodstock. It seen, from the experiment, that
using two different PCR methods, OIE (2006) and Nugen kit, and also histology
analysis showing no WSSV infection in polychaete worms. However, from further
experiment showing that polychaete worm challenge by WSSV have already got
severely stress due to WSSV inocculum treatment, but the level of infection is
still very low so that can not detect by PCR. Shrimp broodstock that have
already fed with WSSV-contaminated polychaete worms for 1 week, based on
clinical signs seems to get infected by WSSV, even the infectivity level is
still very low. It concluded that polychaete worm can be a vector for WSSV in
pond.
Keywords: Polychaete worm,
blackt tiger shrimp broodstock, vector, WSSV
Penulis: Bambang W. Prastowo,
Kade Ariawan, Evy M. Nur, Rahayu Rahardianti, Yuni Setyowati
Kode Jurnal: jpperikanandd090011
