Hubungan Kekerabatan Sapi Aceh dengan Menggunakan Daerah Displacement-loop
ABSTRACT: The aims of this
study were to describe relationship of D-loop of mtDNA Aceh cattle which is
useful database for conducting conservation programme. The whole blood samples
were collected (8 samples for D-loop analysis) from four locations which were
Aceh Besar, Pidie, North Aceh regencies and Banda Aceh city. Out group whole
blood samples were collected from two samples from Bali cattles (Bali Island),
Madura cattle (Madura Island), Pesisir cattle (West Sumatera) respectively and
one sample from PO cattle (West Java). Amplification of D-loop sequences of
mtDNA with BIDLF and BIDLR primary have PCR product 980 bp. The Data were
analyzed using Squint 1.02 and MEGA 4.0 programme. Result of analysis indicate
that Aceh cattle have nearer relationship with zebu and there is items inset of
genetik Bali cattle (Bos javanicus) at the end sequences start ke-354 situs up
to 483, so that the origin Aceh cattle was from Bos indicus which have
hybridization with Bos javanicus.
Keywords: Aceh cattle;
Displacement-loop; DNA; genetic
Penulis: Mohd. Agus Nashri
Abdullah
Kode Jurnal: jppeternakandd080042
