EVALUASI IN VIVO EFEK SINBIOTIK BEKATUL TERFERMENTASI BAKTERI ASAM LAKTAT PROBIOTIK (Lactobacillus plantarum B2 DAN Lactobacillus casei)
ABSTRACT: Rice bran contains
dietary fiber such as hemicellulose, arabinogalactan, arabinoxylan, xylogycan,
proteoglycan, arabinofuranoside, and raffinose. Those dietary fibers have a
potency as prebiotic and to be fermented by probiotic in colon to produce
lactic acid and short chain fatty acid (SCFA). Synbiotic effect of fermented
rice bran by Lactobacillus plantarum B2 and Lactobacillus casei on Wistar rats
(Rattus norvegicus) was investigated. A
two month old Wistar rats (Rattus norvegicus) consisted of four groups (F0, F1,
F2, F3) with five rats of each group were adapted for ten days by giving
standard diet (AIN-93). There were four diet formulas: standard diet of AIN 93
M (F0), standard diet with formulated rice bran media (F1), standard diet with
fermented rice bran media by L. casei (F2) and standard diet with L. plantarum
B2 (F3). The weight, LAB viability, pH, and total acid of rat faecal were
measured in 20 days. SCFA production was measured after 20 days experiment.
Data were analyzed using ANOVA (α=5%) and least significant difference (α=5%).
The best treatment was obtained using ranking method. F3 was the best treatment with fecal LAB
viability of 5.8x107 CFU/g, faecal pH of 6.47 on 20th day experiment, caecum
LAB viability of 6.77x108 CFU/g, caecum total acid of 0.20% lactic acid, caecum
pH of 6,34, and SCFA total of 11.49 mg/g (acetic acid of 7.11 mg/g, propionic
acid of 3.03 mg/g and butyric acid of 1.35 mg/g).
Keywords: synbiotic, rice
bran, probiotic, SCFA, wistar rat
Penulis: Mochamad Nurcholis,
Elok Zubaidah
Kode Jurnal: jppertaniandd110010
