EKSTRAKSI DAN KARAKTERISASI ENZIM POLIGALAKTURONASE ENDOJINUS PADA PULP BIJI KAKAO
ABSTRACT: Endogenous
polygalacturonase (PG) enzymes in cocoa pulp have not been studied yet. The research,
aimed to extract
and characterize endogenous
PG enzymes in
cocoa pulp, was carried
in two steps:
(i) extraction of
endogenous PG enzyme
and (ii) enzyme characterization i.e.
enzyme activity assay
and determination of
enzymatic kinetics (Km and
Vmax), molecular weight
(MW) by electrophoresis, and
determination of its
optimum temperature and pH.
The results showed
that the characteristics of
endogenous PG in
cocoa pulp were
as followed: the relative activity of 4,58± 0,06 Units/ml, Vmax of 6,69 Units/ml, Km of 0,37 %, MW was
32,84 and 63,58 kDa, and the combination of temperature and pH optimum were 42,5oC
and 4,6.
Key words: endogenous
polygalacturonase (PG), cocoa pulp
Penulis: G.P. Ganda Putra,
Harijono, Tri Susanto, Sri Kumalaningsih dan Aulanni’am
Kode Jurnal: jppertaniandd070005
