Efektivitas Penambahan Vitamin E (alfa-Tokoferol) dalam Medium Pencucian Sperma dengan Sentrifugasi terhadap Kualitas Spermatozoa Sapi Brahman
ABSTRACT: The aims of study to
determine the effectiveness of the addition of vitamin E in the washing medium
by centrifugation on sperm quality Brahman cattle. frozen semen of Brahman
cattle, divided into 4 treatment groups addition of vitamin E in the washing
medium: 0.0gr/100 ml medium (K0), 0.1gr /100 ml medium (K1); 0.2gr/100 ml
medium (K2) and 0.3 g / 100 ml medium (K4), each group was repeated 5 times.
Examination of motility, viability and integrity of sperm membrane performed
according to WHO standards. The data obtained were analyzed with one-way ANOVA
and Duncan test. The average percentage of motility, viability and membrane
integrity of spermatozoa in the addition of vitamin E were significantly
different (P <0.05) compared to the control. Percentage of motility,
viability and membrane integrity of spermatozoa in the group K2 significantly
higher (P <0.05) compared with the group K3: K1 and K0. Percentage of
motility, viability and sperm capacitation and sperm live on group K3
significantly higher (P <0.05) compared with the K1 and K0. While the
percentage motility of spermatozoa in the group K1 higher were not significant
(P> 0.05) compared with the group K0. The addition of vitamin E in the
medium on the process of washing spermatozoa Brahman cattle. The addition of
vitamin E 0.2gr/100ml better than vitamin E 0.1gr/100ml and 0.3gr/100ml in
maintaining the percentage of motility and live spermatozoa Brahman cattle.
Keywords: vitamin E;
preparation of sperma; motility; viability and integrity membrane plasma intact
Penulis: Dasrul Dasrul,
Rasmaidar Rasmaidar, Abdul Harris
Kode Jurnal: jppeternakandd120009
