EFEKTIVITAS BERBAGAI WAKTU INJEKSI PGF2Α SEBELUM PENAMPUNGAN SPERMA DOMBA LOKAL TERHADAP KUALITAS SPERMA DALAM PENGENCER TRIS YANG DISIMPAN PADA SUHU 5DERAJAT CELCIUS
ABSTRACT: This experiment
aimed to evaluate the effect of PGF2α and time of injection on the quality and
viability of Local ram sperm in Tris dilution at 50C storage temperature. The
sperm was collected from four Local rams with artificial vagina, once a week
for one month. At the first week, sperm were collected without PGF2α injection;
10 minute after PGF2α injection in second week; 20 minute after PGF2α injection
in third week; and 30 minute after PGF2α injection in fourth week. PGF2α with
dose of 1,0 ml/head (7,5 mg Luprostiol/ml) were given by intramuscular
injection in thigh area. The data were analyzed by Completed Randomized Design.
When there were significant differences continued with Duncan’s New Multiple
Range Test. The result showed that PGF2α injection significantly (p<0.05)
increased the motility sperm and life sperm percentage especially on collected
sperm 10 minute after PGF2α injection. Ejaculate volume, pH, sperm
concentration, abnormal sperm percentation and sperm viability, showed no
significant difference (p>0.05). Based on the result of this study, it was
concluded that PGF2α injection 10 minute before sperm collection could improve
sperm quantity and quality specifically sperm motility and sperm life
percentation.
Key words: local ram,
prostaglandin F2α , quality sperm, dilution
Penulis: Purwaningsih, Sunardi
Kode Jurnal: jppeternakandd100065..
