Analisa Mikrosatelit dalam Bioteknologi Reproduksi Ternak (Suatu Kajian Pustaka)
ABSTRACT: In year 1970 was
found nucleotide sequence which have repeated sequence of nucleotide. with high
polymorh and using PCR could be amplified. That sequenz of nucleotide called
Microsatelite. Microsatelite consist of 1 – 6 repeated nucleotide, which is CA
repeated as mostly a repeated DNA in the animal (Tautz and Renz, 1984). Based
on difference of long and amount of repeated nucleotide, there are three kind
of DNA satelite, midi-, mini- and microsatelite (Matiat and Vergmaud, 1982).
Microsatelite analysis was used to analyze of paternity and identity of animal,
which was done as a conventional analysis with blood group analysis. The
advantage of microsatelite analysis compare to blood group system are the
exclution probability was high (EXP 99.9%), needs small sampel (tissues, sperm
or follicel of hair), could be use for all animal without special age and
possible for died animal.
Keywords: Microsatelite
analysis; paternity; reproductive biotechnology
Penulis: Siti Darodjah Rasad
Kode Jurnal: jppeternakandd090088
