ABSTRACT: Oxidation
process is a natural process which always occurs in fat. The process affects
and decreases the fat quality. Oxidation in fat can be hampered by the addition
of antioxidant. Antioxidant activity of wedusan leaf has to be studied to know
the possibility of wedusan leaf as an antioxidant. Hence, the aims of the
research were to determine the antioxidant activity of ethyl acetate extract of
wedusan leaf using TBA method, and to compare the antioxidant activity of
wedusan leaf and that of BHT. The research method consisted of sample
preparation, extraction, and determination of antioxidant activity using TBA
method. Wedusan leaf was extracted by maceration using n-hexane and ethyl
acetate solvents. The n-hexane extract was 2.90 gram, whereas ethyl acetate
extract was 13.12 gram. Based on qualitative screening on secondary
metabolites, ethyl acetate extract contained flavonoid. The results from GC-MS
indicated that ethyl acetate extract contained methyl heptadecanoic,
methyl-13-octadecenoic, 14,16-octadecadienal, and octadecanoic acid. The order
of inhibition activity of antioxidant were 0.05% (w/v) of BHT > 0.15% (w/v)
of ethyl acetate extract > 0.10% (w/v) of ethyl acetate extract > 0.05%
(w/v) of ethyl acetate extract.
Keywords:
antioxidant, TBA, BHT, Wedusan
Penulis: Purwati,
Undri Rastuti
Kode
Jurnal: jpkimiadd090045
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