ABSTRACT: Calanone is a
natural compound which could be isolated from tree bark of Calophyllum biflorum
Hend and WS ( 0,37 % w/w, crude extract) which have been potency as
antileucemia drug (IC50= 59,4 ug/mL). Based on the research, the compound can
be used as an anti leukemia drug if it value of IC50 < 10 ug/mL. So the aim
of this research are in order to get higher calanone activity by modified of
double bond carbon-carbon and to obtained several compounds as well which has a
higher activity than calanone of its original compound. Several compounds,
several compound can be obtained by modified of doble bond carbon-carbon by
ethylendiamine group and antileukemia test of the new compound calanone
derivatives towards L1210 leukemia cell. The result of the analysis, using thin
layer chromatography with eluent methanol:dichlorometane ( 1:6) , give Rf at
0.51 and initial melting point at range 149oC – 151oC. Infra red
spectrophotometry and mass spectrometry were used to identify the synthesized
product. The result showed that the mass spectra of ethylendiamine calanone
give molecular ion at 449 (M+- H2O, NH3) and ions fragmen at m/e 449; m/e 434;
m/e 393; m/e 105, m/e 77. Infra red spectra of its compound showed new peaks at
1620 cm -1 and 1063 cm -1. The activity value toward leukemia cell was IC50=
46,28 µg/mL and its value higther than calanone origin.
Key word: Calanone,
ethylendiamine calanone, in vitro, Antileukemia
Penulis: Mochamad Chasani ,
Eva Vaulina, Y.D, Ponco Iswanto, M. Hanafi
Kode Jurnal: jpkimiadd080022
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