ABSTRACT: Lipases
are valuable biocatalysts because they act under extremely mild conditions, are
stable in organic solvents, show broad substrate specificity and exhibit high
stereoselectivity. Lipases play important role in various industries such as
detergent, cosmetics, flavor, pharmacy and synthesis of organic compounds. The
increasing of lipases requirements in industries is goading research to get new
lipases resources commited. One of potential lipase resource is Azospirillum
sp.JG3 bacteria from Microbiology Laboratory of Biology Faculty University of
Jenderal Soedirman. The specific targets of this research are to get crude
extract of lipase and investigate its biochemical characteristics. The method
used were rejuvenation of Azospirillum sp.JG3 bacteria, inoculum production,
determination of optimum production time and bacterium growth phase, extraction
and production of lipase to get crude extract, and characterization the
biochemical properties of lipase crude extract. The research resulted that
crude extract of lipase from Azospirillum sp.JG3 had optimum temperature at 40
°C and optimum pH at pH 7. The lipase was a metalloenzyme with Ca2+ as its
cofactor. The lipase was stable in three organic solvents tested, (chloroform,
n-hexane and ether).
Keywords:
Azospirillum sp. JG3, lipase activity, biochemical characteristics
Penulis: Puji
Lestari, Santi Nur Handayani dan Oedjijono
Kode
Jurnal: jpkimiadd090047
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