ABSTRACT: Antibacterial
compound from methanol fraction of methylene dichloride extract of pelawan bark
had been studied. Pelawan bark was extracted using ethanol 95% and CH2Cl2-water
(1:1), respectively. Extract of CH2Cl2 was partitioned using hexane-methanol
90% (1:1). Afterwards, methanol fraction was analyzed by thin layer
chromatography methods to find the best eluent for column chromatography. The
best eluent came from mixture of acetone-chloroform-methanol (0,6:4:0,4). The
fractionation yielded 16 fractions with the first (M1) and the third (M5)
fractions became the one spotted fractions. M1 and M5 fractions were examined
for their antibacterial activity using paper diffusion methods by determining
minimum inhibitory concentration value. M1 fraction showed greater activity
than M5 fraction with minimum inhibitory concentration value of 2,7857 mg/mL.
The result of phytochemistry test showed that both fractions contain terpenoid.
Keywords: pelawan, antibakteri
Penulis: Purwantiningsih
Sugita
Kode Jurnal: jpkimiadd070026
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