ISOLASI DAN UJI POTENSI BAKTERI TANAH PERTANIAN BERASTAGI SUMATERA UTARA DALAM MENDEGRADASI FUNGISIDA ANTRACOL BERBAHAN AKTIF PROPINEB
Abstract: Isolation and
potential Berastagi agricultural
soil bacteria, North
Sumatra in degrading
propineb-based antracol
fungicide has been
conducted. The isolated
bacteria were grown
on Bushnell Hass
Broth (BHB) containing 2%
of propineb-based antracol
fungicide with propineb
as the active
compound. The cultures have been grown on shaking incubator
at 150 rpm for 21 days. The media BHB containing 2% of propineb-based antracol
fungicide without bacteria was used
as a control.
The parameters observed were the
growth bacterial,
biosurfactant activity, biosurfactant
concentration and the
residues of propineb
which were observed on day 0th,
7th, 14th and 21th. A total of sixteen
bacterial isolates were isolated using selective media Bushnel Hass
Agar (BHA) containing
2% propineb-based antracol
fungicide. Two bacterial
isolates which were CBA 02 and
JBA 04 were selected for further test to determine their ability to degrade
propineb. JBA 04 showed much
higher ability in
reducing propineb
concentration up to 60.86%,
while CBA 02
was only 5.59% than that of
control.
Keywords: biodegradation,
bioremediation, biosurfactant, fungicides, propineb
Penulis: Diah Sri Utami, Nunuk
Priyani dan Erman Munir
Kode Jurnal: jpbiologidd130004
