ABSTRACT: Sirih duduk or Piper
sarmentosum Roxb. ex Hunter have been long used the people in Indonesia for
traditional medicine to cure various diseases. One of them was used to treat
fungoid diseases. This research was aimed to examine the anticandidaisis
activity from extract of P. sarmentosum bark, isolate and identify
anticandidaisis compounds from P. sarmentosum bark. The isolation of bioactive
compounds of P. sarmentosum bark was performed by extraction the powder of P.
sarmentosum bark using methanol. The methanol extract was then fractionated
using n-hexane, chloroform and ethylasetat. The fractions respectively was
examined their activity against C. albican. The active extract was then
fractionated performed by coloumn chromatography and was then separated
performed by thin layer chromatography. Identification of bioactive compound
was carry out using gas chromatography-mass spectrometry (GC-MS). The results
show that chloroform, ethylasetat and methanol extracts of P. sarmentosum have
more activities against C. albicans than miconazol. Bioactive compound from
extract of P. sarmentosum bark was bis(2-ethylhexyl)phtalat.
Key word: Piper sarmentosum,
bioactivity test, C. albicans, phtalat
Penulis: Suwandri, Hartiwi
Diastuti, Purwati
Kode Jurnal: jpkimiadd070019
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