ABSTRACT: Sirih duduk or Piper
sarmentosum Roxb. ex Hunter have been long used the people in Indonesia
for traditional medicine
to cure various
diseases. One of
them was used
to treat fungoid diseases.
This research was
aimed to examine
the anticandidaisis activity from
extract of P.
sarmentosum leaves and
to isolate and
identify the anticandidaisis compounds from
P. sarmentosum leaves.
The isolation bioactive
compounds of P. sarmentosum leaves
was performed by
extraction the powder
of P. sarmentosum
leaves using methanol and
the bioactivity tests
were performed against
Candida albicans. The methanol extracts was then fractionated
using organic solvents such as n-hexane, benzene, chloroform, and
ethyl acetate. Identification of
the bioactive compounds
was carried out using
ultraviolet-visible
spectrometry, fourier transform
infra red spectrometry
and gas chromatography-mass spectrometry.
The result showed
that the chloroform
fraction of methanol extract
of P. sarmentosum
leaves have activity
against C. albicans.
The fractionation was performed
by coloumn chromatography using
n-hexane-ethyl acetate(1:1)
eluent. A bioactive
compound of sinamic
acid derivative was
4-ethoxy-2-hidroxy-3,5-dimethoxy sinamic acid could be purely isolated
Keywords: Piper sarmentosum,
bioactivity test, C. albicans
Penulis: Suwandri, Hartiwi
Diastuti
Kode Jurnal: jpkimiadd060026
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