ABSTRACT : Calanone is anticancer
compound that is isolated from species Calophyllum.
The biological activity of calanone againts leukemia cell L1210 is relatively
low showed by IC50 value
at 59.4 μg/mL. A compound have a high biological activity if the value of IC50under
10 μg/mL. This research aimed to obtained calanone hydrat compound which has a higher
activity to inhibit leukemia L1210 cancer cell growth. Calanone hydrat compound
is got through with two step reaction, first step was protonated double bond by
acetic acid reagent and the second step is nucleophilic addition by water.
Initial analysis for the synthesize product compound is done with thin layer
chromatography (TLC) using n-hexane and ethyl acetate 2:1 (v/v) eluent. Coloum
Chomatography and recrystallization is used to pure the synthesize product
compound with n-hexane and methylen chloride 1:1 (v/v) solvent. The result
analyzed by thin layer chromatography. The synthesized product give Rf at
0.4545 and rendement 4.72% (w/w), forming of brownish yellow chromatic powder
and melting point at 190oC-192oC. Identification
structure of synthesize product compound is done with mass spectrometer. The
result of mass spectrometer identification were moleculer ion M+-18
(M+ 424) with
fragments m/e=409, m/e=395, m/e=381, m/e= 331, m/e=317, m/e=303, me/=176,
m/e=105, and m/e=77. Citotoxicity test againts leukemia cell L1210 yield the
value IC50 equal to
45.64 μg/mL.
Keywords: calanone hydrat,
anti cancer, leukemia cell L1210
Penulis:
Mochamad Chasani, Eva Vaulina, Ponco Iswanto, Yayu Rahayu
Kode
Jurnal: jpkimiadd100044
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