FERMENTASI SUBSTRAT PADAT DAN FERMENTASI SUBSTRAT CAIR PADA PRODUKSI ASAM LAKTAT DARI KULIT PISANG DENGAN RHIZOPUS ORYZAE
Abstract: Indonesia is one of
the major banana producing countries in the world. There are many varieties of
banana grown in Indonesia. While the fruit is valuable for consumption, banana
peels mostly are discarded as waste. In fact, banana peels are high in
nutrition, thus they are potential to be converted into other valuable products
such as lactic acid. The objective of this research was to study the effect of
fermentation methods, i.e. solid substrate fermentation and liquid substrate
fermentation, in the production of lactic acid from banana peel waste by the
fungus Rhizopus oryzae. There were three steps involved in this research: (1)
preparation of fermentation medium, (2) banana peel fermentation, and (3)
lactic acid purification and recovery by using Amberlite IRA-400 resin. In
solid substrate fermentation, dried banana peels were firstly crushed, while in
liquid substrate fermentation, fresh banana peels were extracted by water.
Following this, banana peel powder and extract banana peel were inoculated with
Rhizopus oryzae and incubated at 30 oC for 144 h. As results, the yield of lactic
acid produced from solid substrate fermentation (0,79 g lactic acid/g starch)
was higher than that from liquid substrate fermentation (0,15 g lactic acid/g
starch).
Keywords: lactic acid; liquid
fermentation; solid state fermentation; banana peel; Rhizopus oryzae
Penulis: Ery Susiany
Retnoningtyas, Aning Ayucitra, Fandy Maramis, Ong Wei Yong, Frengky W. Pribadi
dan Nelsi K. Tanti
Kode Jurnal: jpkimiadd130119
