Abstract: This research was aimed to investigate the antioxidant activity
of purple sweet potato leaves cultivated
in Saree, Aceh
Besar. The study
was conducted in
two stages. The
first stage included sampel
preparation, phytochemical test, λ max determination, testing of antioxidant activity by
reducing power method,
and TLC analysis.
In stage 2
purification using column chromatography, phytochemical
test, and antioxidant
activity test were
performed. The sample was
dissolved by using
70% ethanol and
1.5 N HCl
with a ratio
of 85:15 (v/v). Phytochemical test
results showed that
the sample consisted
of not only
flavonoids but also tannins.
The Rf value
for both sample
and standard are
respectively 0.55 and
0.61 with absorbance value
of 0.826 and
0.845. The sample
was then purified
using column chromatography and
25 fractions were
grouped based on
the color intensity
abbreviated as FA, FB
and FC. Photochemical
test was then
conducted to examine
the purity of
obtained fraction and FB was found to be relatively purer than other
fractions. Reducing power test of FB
with variation of
weight 10 mg,
20 mg, 30
mg, 40 mg,
and 50 mg
gave the percentage inhibition 64.1,
6.7, 67.9, 70.3
and 73.6, respectively,
while the percentage
value of α- tocopherol
at simmilar weight
variation are 32.1,
36.7, 43.5 and
50.2%. From the
result obtained, it can be
concluded that the
antioxidant activity of
ethanol extract of
thick purple sweet potato leaves
is higher than α-tocopherol.
Keywords: antioxidant activity, purple sweet potato leaves, reducing
power
Penulis: Sulastri, Erlidawati, Syahrial, Muhammad Nazar, Thursina
Andayani
Kode Jurnal: jpkimiadd130112
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