ABSTRACT: The mangrove plant
have been long used the people for traditional medicine to cure various
diseases, one of them to cancer therapy. Investigation the anticancer potent of
R. mucronata has not been carried yet. This research was aimed to toxicity test
of R. mucronta leaf extracts againts Artemia salina Leach, then cytotoxicity
test of R. mucronata leave extracts againts Raji cancer cells. The extraction
of R. mucronata leaf was peformed by maseration with ethanol. The ethanol
extracts was partitioned with chloroform, ethylacetate and methanol. The
ethanol extracts of R. mucronata leaf respectively was examined their toxicity
againts A. salina Leach larv. The toxic exctracts was examined their
citotoxicity againts Raji cells. The ethanol extract of R. mucronata leaf has
toxic character because has LC50 < 1000 mg/mL. Toxicity test againts A.
salina and citotoxicity test againts Raji cells showed that the chloroform fraction
of ethanol extract of R. mucronata leaf have highest activity, respectively
IC50 value equal to 290,92 mg/mL and 105,56 mg/mL. Phytochemical study showed
that the active fractions contained flavonoid, terpenoid and alkaloid.
Keywords: R. mucronata,
anticancer, Artemia salina, Raji cells
Penulis: Hartiwi Diastuti,
Warsinah, Purwati
Kode Jurnal: jpkimiadd090053
Artikel Terkait :
