The use of coconut pulp as a feed substrate to methanogenesis inhibitor in in vitro rumen fluid fermentation
Abstract: This experiment was
conducted to determine the effect of the adding of coconut pulp in ruminant
feed on methane reduction in vitro. Rumen fluid and substrate of forage and
concentrate in ratio 60:40 were in this study. This experiment consisted of
five treatments of coconut pulp as followed: R1: feed substrates without the
coconut pulp; R2: substrate feed with coconut pulp 5%; R3: substrate feed with
coconut pulp 10%; R4: Substrate feed with coconut pulp 15% and R5: substrate
feed with coconut pulp 20%. Each treatment was conducted to four replications.
Fermentation was done using the Hohenheim Gas Test (HGT) incubated at 39oC for
72 hours. At the end of fermentation was determined production of methane,
parameters of fermentation, microbial activity, feed digestibility and fatty
acids profile of the ruminal fluid. Data obtained were analyzed by using
analysis of variance using completely randomized design. The differences of
mean values were analyzed by Duncan multiple range test (DMRT). The results
showed that methane production has decreased about 13.74%-17.39% when the feed
was given coconut dregs 15-20%. Based on the results of the study was concluded
that the supplementation of coconut pulp up to 15-20% of 100% dry matter can be
used as a substrate of ruminant feed, because it can reduce methane and does
not interfere the microbial activity in the fermentation of rumen fluid through
in vitro. The supplementation of coconut pulp can be a referencein designing
the methanogenic inhibitor feed.
Keywords: coconut pulp; gas
tes; methane; parameter of fermentation; ruminal fermentation
Author: E. H. B. Sondakh, J.
A. D. Kalele, F. S. Ratulangi
Journal Code: jppeternakangg170022
