Sintesis protein mikroba dan aktivitas selulolitik akibat penambahan level zeolit sumber nitrogen slow release pada glukosa murni secara in vitro
Abstract: This research aimed
to determine the effect of the level of zeolites as slow release nitrogen in
pure glucose on microbial protein synthesis and cellulolytic activity. The
material used were zeolite of clinoptilolite type with size 40-60 mesh, urea,
pure glucose, cow's rumen fluid from RPH Ungaran. The research used completely
randomized design (CRD) with 5 treatments and 4 replications. The treatments
were T1, T2, T3, T4 and T5 = pure glucose + additional level 2%, 4%, 6%, 8% and
10% of zeolite slow release respectively. Variables observed were microbial protein
synthesis and cellulolytic activity. The results showed that the treatment
significantly (P<0.05) increased microbial protein synthesis and
cellulolytic activity. Results of protein synthesis from higher to lower number
were T2 (59.50 mg/ml), T3 (55.58 mg/ml), T4 (47.55 mg/ml), T5 (42.82 mg/ml) and
T1 (31.68 mg/ml) respectively. Results of cellulolytic activity from higher to
lower number were T5 (3.55 mol ml-1 min-1), T4 (2.36 mol ml-1 min-1), T2 (2.23
mol ml-1 min-1), T3 (2.16 mol ml-1 min-1) and T1 (2.09 mol ml-1 min-1)
respectively. The results of microbial protein synthesis and cellulolytic
activity at each treatment were in normal level. The addition of the zeolite
level as slow release nitrogen source up to 4% increase microbial protein
synthesis.
Keywords: Zeolites, microbial
protein synthesis and activity of cellulolytic
Penulis: Al Qori'ah, Surono, Sutrisno
Kode Jurnal: jppeternakandd160123
