Rasio Spermatozoa X:Y dan Kualitas Sperma pada Kambing Kacang dan Peranakan Ettawa
Abstrak: The objective of the
study was to know the difference of X:Y spermatozoa ratio and sperm quality of
Kacang and Ettawa-crossed breed goats. It was conducted at Laboratory of Animal
Physiology and Reproduction, Faculty of Animal Science Universitas Gadjah Mada
for three months, starting December 2009 to February 2010. Materials of the
study were sperm collected from six two-year Ettawa-crossed breed goats
weighing 31.5±2.2 kg and from six two-year Kacang goats weighing 18.0±2.1 kg.
The sperm was collected using artificial vagina with frequency of twice a week
for each goat and it was repeated eight times. The quality and quantity of the
sperm was immediately evaluated by its volume, concentration, motility,
viability and abnormality. To determine the ratio of X:Y spermatozoa, smear
preparation was made and spermatozoa head was then measured using Scion Image
software. The results of the measurement were used for identification of X or Y
spermatozoa. Spermatozoa having bigger head than the average were identified as
X and those having smaller head as Y. The result showed that the ratio of X:Y
spermatozoa of Ettawa-crossed breed goats (50.6±1.8:49.4±1.8 %) was not
significantly difference with that of Kacang goats (49.7±1,7:50.3±1.7 %). Sperm
volume of Ettawa-crossed breed goats (1.00±0.2 ml) was higher than that of
Kacang goats (0.62±0.2 ml). Sperm concentration of Ettawa-crossed breed goats
(2,865±431 million/ml) was not significantly difference compared to that of
Kacang goats (2,840±383 million/ml). Spermatozoa motility of Ettawa-crossed
breed goats (66.7±9.8 %) was higher (P<0.05) than that of Kacang goats
(50.0±7.1 %). Spermatozoa viability of Ettawa-crossed breed goats (80.0±7.1 %)
was higher (P<0.05) than that of Kacang goats (67.5±9.4 %). Spermatozoa
abnormality of Ettawa-crossed breed goats (8.2±3.3 %) was not significantly
difference compared to that of Kacang goats (8.6±2.4 %). The study was
concluded that the breeds of Ettawa-crossed breed and Kacang goats have
indifferent ratio of X:Y spermatozoa, while the sperm volume, motility and viability
of Ettawacrossed breed goats was better than that of Kacang goats.
Key words: XY spermatozoa,
quality and quantity, Ettawa-crossed breed, Kacang
Penulis: S Bintara
Kode Jurnal: jppeternakandd110232
