POLYMORPHISM STEAROYL-COA DESATURASE (SCD) GENE AND ASSOCIATON WITH CHARACTERISTICS MEAT IN BALI CATTLE
Abstract: Stearoyl-CoA
desaturase (SCD) is an enzyme produced by SCD gene which is responsible for a
conversion of saturated fatty acids (SFA) to mono-unsaturated fatty acid (MUFA)
in adipose tissue. This enzyme affects the fats in intramuscular so having
influence on marbling. The purpose of this study was to obtain the
polymorphisms of the SCD gene and their associations with meat quality traits
in Bali cattle. The number of samples used were 48 heads of cattle consisted of
24 bulls and 24 cows from BPTU-HMT Bali cattle in the province of Bali. The SCD
gene has been amplified using forward primer 5’-ACC CCT TGG TGT GTG GTT GTT CTT
C-3 ‘and reverses primer 5'-CCT GAC GAT ACT ATG TTT CTA CTT C-3'. The
polymorphisms of the SCD gene were identified by direct sequencing method. Meat
quality traits such as thick of longissimus dorsi (TLD), thick of back fat
(TBF), thick of fat rump (TFR), thick of rump (TR), marbling score (MS), and
the percentage of intramuscular fat (PIMF) were analyzed using the Veterinary
Ultrasound Scanner. To determine Hardy-Weinberg equilibrium status, both allele
and genotype frequencies were analyzed using GENEPOP program (V3.2).
Association of the SCD gene SNP and meat quality traits was analyzed by GLM.
This result showed that there were 5 monomorphic SNPs (c.10153A>G, c.10318C>A,
c.10329C>T, g.10394G>A, g.10486A>C) and 3 polymorphic SNPs
(g.10360G>A, g.10428C>T, g. 10487G>A) were in HW equilibrium.
Association analysis showed that g.10428C>T SNP significantly affected
marbling score (MS) and percentage of intramuscular fat (PIMF) (P<0.05).
Based on these results, g.10428C>T SNP of the SCD gene may be used as a
candidate marker to select meat quality traits in Bali cattle.
Keywords: Bali cattle; SCD
gene; SNP
Author: A. Alwiyah, H.
Naraini, P. P. Agung, J. Jakaria
Journal Code: jppeternakangg170006
