Pemanfaatan casa dalam observasi motilitas spermatozoa semen cair Sapi Madura dalam pengencer berbeda
Abstract: The purpose of this
study was to measure the motility of Madura bull spermatozoa using three
different diluents (tris aminomethane, CEP-2 andskim milk) duringcold storage.
The research material used were 2 Madurabulls. Collecting semen method used
artificial vagina, followed by freshsemen analysis and processing of liquid
semen. Observation of liquid semenquality was carried out up to the 5th days of
cold storage. Motilityexamination of semen liquid used Computerized Assisted
Semen Analysis (CASA), namely SCA v.5.2. The parameters measured were:
progressive motility, motility, velocity straight linear (VSL), velocity curve
linear (VCL),velocity average pathway (VAP), straightness (STR), linearity
(LIN), wobble(WOB), hyperactivity (H), Beat cross frequency (BCF), amplitude
lateral head(ALH). Data was analyzed by Minitab 17. Motility and progressive
motility ofspermatozoa in tris aminomethane and CEP-2 were higher than skim
milk for 5 days of cold storage. The VCL value of tris aminomethane was higher
thanCEP-2 and skim milk. VSL scores barely show the difference among diluents. VAP
values in tris aminomethaneand CEP-2 are higher than skim milk at thebeginning
of storage. The LIN, STR and WOB values of the CEP-2 and skim milkyield higher
values than tris aminomethane. The percentage value of hyperactivity
spermatozoa was same among diluents. The ALH value oftrisaminomethane was
higher than CEP-2 and skim milk. BCF values in CEP-2was higher than skim milk
and tris aminomethane. Tris aminomethane and CEP-2 can support the motility of
Madura bull spermatozoa than skim milkfor 5 days of storage
Keywords: motility;Madura
bull;CASA
Penulis: Dian Ratnawati, Nurul
Isnaini, Trinil Susilawati
Kode Jurnal: jppeternakandd170064
