Kualitas spermatozoa semen beku sapi Simental dengan suhu dan lama thawing yang berbeda
Abstract: This study aims to
investigate the quality of Simental frozen semen based on the temperatures and
duration of thawing. The materials used in this study were frozen semen of
Simental cattle. The study used a laboratory experiment using a completely
randomized design (CRD) factorial with 6 treatments and 10 replications. The
thawing temperatures were 37°C and 25°C and the durations of thawing were 7
seconds, 15 seconds and 30 seconds. The variables observed in this study were
the motility, viability and abnormality of Simental frozen semen. The data were
analyzed using analysis of variance (ANOVA). The difference in advanced test
was done by using the smallest Real Difference Test. The results showed that
the temperature and duration of thawing and the interaction of temperature and
duration of thawing provide a highly significant difference (P <0.01) on
motility, viability and abnormality of Simental frozen semen. The highest
motility (45,5%) and viability (75,65%) were found on 37°C and during 30
seconds of thawing, and the lowest abnormality was 4,71%. This study concludes
that the optimal thawing temperature and the lenght of thawing for Simental
frozen semen is 37°C and 30 seconds respectively.
Keywords: thawing, sperm,
motility, viability, temperature, abnormality
Penulis: E. D. Kusumawati, A.
T. N. Krisnaningsih, R. R. Romadlon
Kode Jurnal: jppeternakandd160138
