Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken
Abstract: Primordial germ
cells (PGCs) are cells that will differentiate themselves into spermatogonia in
the testis or oogonia in the ovary. Primordial germ cells arise from epiblast
and circulate through the bloodstream and finally entering gonadal anlage. The
aim of this study was to determine the number of gonadal PGCs of KUB chicken at
different development stages. Sixty KUB chicken fertile eggs were divided into
four groups (6, 7, 8, and 9 days incubation periods), and incubated at 38 oC
with a humidity of 60%. Harvesting was synchronized to the embryonic
development at 6-9 d. Gonads were collected using sharp tweezers, and were
placed in Eppendorf tube 1.5 mL containing 500 µL PBS [-]. Gonadal PGCs were
purified using PBS [-]. The results showed that the average number of gonadal
PGCs at 6, 7, 8, and 9 d were 113.7; 143.5; 92.9; and 85.7 cells per embryo,
respectively. Number of gonadal PGCs per embryo of KUB chicken were
significantly affected by stage of embryonic development (P<0.05), which
reached a peak at day 7 of incubation, so that the isolation and collection of
PGCs from the gonads were recommended at day 7 of incubation. This information
is useful in production of germline chimera of other Indonesian local chickens.
Keywords: KUB chicken; embryo
development; gonad; PGCs
Author: Soni Sopiyana, M. A.
Setiadi, M. Fahrudin, I. Supriatna
Journal Code: jppeternakangg170051
