Isolation and Characterisation of Sulphur Oxidizing Bacteria Isolated from Hot Spring in Malaysia for Biological Deodorisation of Hydrogen Sulphide in Chicken Manure
Abstract: In this study, the
isolation of sulphur oxidising bacteria (SOB) from hot spring in Malaysia was
carried out in an enrichment culture using sodium thiosulphate as a sole energy
and CO2 as a sole carbon source. A total number of 80 SOB isolates were
obtained from the agar plate and considered as positive SOB due to their
abilities using thiosulphate for growth. All the isolates were initially
screened for their fast growths in liquid medium and 13 isolates were selected
for another screening process. Three SOB isolates namely isolate AH18, AH25,
and AH28 were selected based on their abilities to grow faster, produce the
highest sulphate ion and reducing the pH in the growth medium. The cells were
Gram-negative and short rod-shaped. The effects of various variables including
temperature (25-45 °C), pH (4-9), sodium thiosulphate concentrations (4-100 mM)
and metabolic characteristic were evaluated on bacterial growth and their
sulphur oxidation activities. The optimum pH of all the potential isolates
occurred at pH 8.0. Meanwhile, the optimum temperature for isolate AH18, AH25
and AH28 occurred at 45 °C, 30 °C, and 30-45 °C, respectively. The three
isolates were classified as facultative chemolithotroph with the capability of
growth in thiosulphate concentration as high as 100 mM. Therefore, given the
ability in the oxidation of thiosulphate, temperature and pH adaptabilities,
with the metabolic flexibilities of isolates AH18, AH25, and AH28 could be a
good H2S biological deodorizing candidate.
Keywords: sulphur oxidising
bacteria; hot spring; biological deodorisation; chicken manure; hydrogen
sulphide
Author: M. Y. Hidayat, H. M.
Saud, A. A. Samsudin
Journal Code: jppeternakangg170072
