Insulin-Like Growth Factor-I Concentration in the Follicular Fluid of Bali Cattle and Its Role in the Oocyte Nuclear Maturation and Fertilization Rate
Abstract: The objective of
this study was to determine the concentration of IGF-I in the follicular fluid
(FF) of Bali cattle and its role in the nuclear maturation and fertilization
rate. The follicular fluid was collected by the aspiration technique, then it
was centrifuged at 1500 g for 30 min at 24oC. The supernatant was collected and
stored at -20oC until being used in the experiment for analysis of IGF-1. A
total of 1105 oocytes were used in this study. The oocytes were matured in M199
without supplementation of bovine serum albumin, with supplementation of BSA,
and with supplementations of 10% FF (v/v) from the follicle with diameter
Ø<4 mm, 4≤Ø<6 mm, 6≤Ø<8 mm, and Ø≥8 mm at the luteal phase and then
fertilized. The results showed that the concentrations of IGF-I in the FF
obtained during the luteal phase was significantly higher (P<0.05) compared
to those obtained during follicular phase. The IGF-I concentrations in the
follicular fluid of follicle with diameter smaller than 6 mm were significantly
higher (P<0.05) compared to those with diameters larger than 6 mm. The
percentage of nuclear maturation rate of oocytes cultured with FF obtained from
follicle with diameter <4 mm was significantly higher (P<0.05) compared
to those obtained from the other groups of follicle diameters. The
supplementation of maturation media with BSA and FF were able to improve
fertilization rate significantly (P<0.05) compared to maturation media
without BSA. In conclusion, the concentration of IGF-I in the follicular fluid
obtained during the luteal phase was higher compared to those obtained during
the follicular phase. The IGF-I concentrations in the follicular fluid of
smaller follicles (diameter <6 mm) were higher compared to those in the
large follicles (diameter ≥6 mm). The supplementation of FF can improve the
nuclear maturation and fertilization rate
Keywords: IGF-I, luteal,
follicular, Bali cattle, oocytes
Author: H. Hasbi, S. Gustina,
N.W. K. Karja, I. Supriatna, M. A. Setiadi
Journal Code: jppeternakangg170049
