IDENTIFIKASI KERAGAMAN GEN BMPR-1B (Bone Morphogenetic Protein Receptor IB) PADA AYAM ARAB, AYAM KAMPUNG DAN AYAM RAS PETELUR MENGGUNAKAN PCR-RFLP
Abstract: BMPR-1B gene closely
related to the rate of ovulation and follicular maturation. In poultry, BMPR-1B
gene is located on chromosome 14th consists of 13 exons. BMPR-1B was expressed
in the granulosa cells of the ovary and teca interna poultry. BMPR-1B was able
to accelerate the maturation of the follicles so that the ova produced
increasingly. BMPR-1B gene diversity was associated with poultry
production. DNA isolation was performed
using GeneJET Genomic DNA Purification Kit from blood samples derived from 28
native chickens, 20 layer chickens and 10 arab layer chickens. A total of 58
DNA were used as a template in the
process of BMPR-IB gene amplification using Polymerase Chain Reaction method.
Primers used in this study according to Zhang et al. (2008), Forward primer 5
'ATG GCT GGG AAG TCT GGA TG 3'dan Reverse primer 5' TGC CTT CTG TGT TAA CCG C 3
'with a length of 581 bp. This primary amplify BMPR-1B gene segment from exon 6
to exon 7 including the entire intron 6 with an annealing temperature of 58 0 C.
BMPR-IB gene diversity was detected using restriction enzymes HindIII were
incubated for 2 hours. The results of the study was showed that Hind III
restriction enzymes do not cut the BMPR-1B gene segments in the population of
native chickens, arab layer chickens and layer chickens, they were monomorphic.
Keywords: BMPR-1B gene,
PCR-RFLP, native chicken, arab layer chicken,layer chicken
Penulis: Hidayati, Eniza
Saleh, Tahrir Aulawi
Kode Jurnal: jppeternakandd160210
