IDENTIFICATION OF SNP SPESIFIC MARKER FOR CRUSTACEAN HYPERGLYCEMIC HORMONE GENE: A SOMATIC GROWTH-RELATED IN GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii)
Abstract: High size variation
of giant freshwater prawn was found in harvest and resulting in low
productivity. Marker assisted selection may be useful to generate broodstock
that produces progeny with high growth and homogeneity. This study was
conducted to obtain growth related molecular marker in giant freshwater prawn.
Genomic DNA was extracted from swimming leg (pleiopods) of 10 giant freshwater
prawns fifth Generation for existence of SNP identification, consisted of 5 fast
growth (FG) and 5 slow growth (SG).
While for SNP confirmation and resolving power of specific primer
studies. The pleiopods sample was taken from six generation of 201 giant
freshwater prawns, consisted of 129 fast-growth (FG) with 16.06 ± 2.48 g body weight
and 72 slow-growth (SG) with 6.05 ± 0.90 g body weight. Oligonucleotide primers
were designed according to Gene Bank database of crustacean hyperglycemic
hormone (CHH) gene sequence. The amplified DNA fragment was then sequenced. The
results of sequencing showed there was one base different in nucleotides of FG
and SG prawns. Six set of primers were designed based on those CH gene
sequence. PCR analysis resulted one set of primers which showed a specific
amplification product of 280 bp for growth. The result of sequence analysis
using the basic local alignment search tools showed that the nucleotide
sequence of those PCR products had similarity of 99%-100% with CHH gen of M.
rosenbergii. Thus, a candidate of growth related molecular marker have been identified
for giant freshwater prawn.
Keywords: giant freshwater
prawn; SNP molecular marker; fast growth; slow growth
Author: Asep Sopian, Alimuddin,
Imron Imron, Harry Krettiawan, Fajar Anggraeni, Desy Nurul Astuti
Journal Code: jpperikanangg170020
