Genetic polymorphism of kappa-casein gene in Friesian Holstein: a basic selection of dairy cattle superiority
Abstract: Caseins are milk
protein subdivided into four main groups which are αS1, αS2, β-casein and
kappa-casein (CSN3). Kappa-casein gene influences the manufacturing of milk
properties. The aim of this study was to identify the kappa-casein gene
polymorphism in Friesian Holstein (FH) cattle. Fifty nine (59) samples
consisted of 32 (Malang), 10 (Sukahati Bogor) and 17 (Research Center for
Biotechnology-Indonesian Institute of Sciences’s collections)were applied in
this study. DNA samples were extracted by high concentrated NaCl and quantified
by spectrophotometer. The kappa-casein gene was amplified at 379 bp fragment by
PCR method using a pair primer of kappa-casein at 56oC annealing for 30 cycles.
PCR-RFLP technique with HindIII was used for genotyping analysis. The result
showed that there were three variants of genotypes (AA, AB and BB) in two
populations from Malang and RC for Biotechnology-LIPI’s collection while cattle
from Sukahati had only AA and AB genotypes. The averages of genotype
frequencies were 65.28%; 65.28%; and 3.00% for AA, AB and BB genotypes
respectively while frequencies of 0.81 and 0.19 were for A and B alleles,
respectively. FH cattle populations were in equilibrium genetics. This finding
concludes that polymorphism was found in three of FH populations with A allele
was more common in kappa-casein locus. B allele is known having association
with milk production, milk component and cheese yield. Increasing of B allele
would influence on milk performance of FH cattle. Explorations of quantitative,
qualitative and molecular genetics are important to improve dairy cattle
performance.
Keywords: kappa casein gene;
polymorphism; Friesian Holstein; dairy cattle
Author: S. D. Volkandari, I.
Indriawati, E. T. Margawati
Journal Code: jppeternakangg170040