Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross
Abstract: Stearoyl-CoA
desaturase (SCD) is an integral membrane protein of endoplasmic reticulum (ER)
that catalyzes the rate limiting step in the monounsaturated fatty acids from
saturated fatty acids. Selection for fatty acids traits based on molecular
marker assisted selection is needed to increase a value of chicken meat. This
study was designed to analyze expression and associations of SCD gene
polymorphisms with fatty acid traits in F2 kampung-broiler chicken cross. A
total of 62 F2 kampung-broiler chicken cross (29 males and 33 females) were
used in this study. Fatty acid traits were measured at 26 weeks of age. Samples
were divided into two groups based on fatty acid traits (the highest and the
lowest). Primers in exon 2 region were designed from the genomic chicken
sequence. The SNP g.37284A>G was detected and polymerase chain
reaction-restriction fragment length polymorphism (PCR-RFLP) method was then
used to genotype. The expression of SCD gene was analyzed using quantitative
real time PCR (qRT-PCR). The result showed that there were three genotypes (AA,
AG, and GG) found in this study. The SCD|AciI polymorphism was significantly
associated with palmitoleic acid (C16:1), fatty acids total and saturated fatty
acid in 26 weeks old of F2 kampung-broiler chicken cross (P<0.05). The SCD
gene was expressed for polyunsaturated fatty acids in liver tissue in two
groups of chickens. In conclusion, the SCD gene could be a candidate gene that
affects fatty acids traits in F2 kampung-broiler chicken cross.
Keywords: kampung-broiler
chicken cross; fatty acid; SCD gene; polymorphism; expression
Author: A. Furqon, A. Gunawan,
N. Ulupi, T. Suryati, C. Sumantri
Journal Code: jppeternakangg170069
