Exploration, Isolation, and Identification of Carotenoid from Bacterial Symbiont of Sponge Callyspongia vaginalis
Abstract: During the past two
decades research on marine bacteria has highlighted the tremendous potential of
symbiotic-microorganisms as a source of bioactive secondary. One of the
potential of the bacterial symbionts is producing a natural pigment, and these
organisms can be used as a sustainable source of natural pigments. Carotenoid
is one of the most important pigments that has important roles in physiological
and molecular processes of microorganisms, as well as for human health. The
objective of this study is to analyze carotenoid pigments from marine bacterial
symbionts from sponge and to identify bacterial symbionts that produce
carotenoid pigments. Pigment analysis was performed by a UV-VIS
spectrophotometer and High Performance Liquid Chromatography (HPLC). Molecular
bacterial identification was performed based on 16S rDNA sequence. The
isolation of bacterial symbionts from C. vaginalison Zobell 2216E medium
resulted in one bacterium, CB-SP5, positively synthesized carotenoids. By
reverse phase HPLC analysis, the carotenoid pigments in the bacterial symbionts
were identified as diadinoxanthin, fucoxanthin, neoxanthin, dinoxanthin,
anddiadinochrome. CB-SP5 shared the highest level of 16S rDNA gene sequence
similarity with Psychrobacter celer (99%).
Keywords: carotenoid, sponge,
bacterial symbiont, 16S rDNA.
Author: Iqna Kamila Abfa, Ocky
Karna Radjasa, A B Susanto, Handung Nuryadi, Ferry F. Karwur
Journal Code: jpperikanangg170044
