DAYA HIDUP SPERMATOZOA KAMBING PERANAKAN ETAWA YANG DIPRESERVASI DENGAN BERBAGAI JENIS PENGENCER
Abstract: The purpose of this
research was to examine the effectivity of Tris, sodium citrate, and lactose
extenders in maintaining viability of etawa crossbreed (EC) goat spermatozoa
preserved at 3–5oC. Semen was collected
using artificial vagina once a week for six times as replicate. Fresh semen was divided into three tubes then
diluted with Tris, sodium citrate, and lactose extenders, respectively. Diluted-semen were stored in refrigerator at
3–5oC. Quality of diluted-semen
including percentages of motile spermatozoa (MS) and live spermatozoa (LS) were
evaluated every day during storage at 3–5oC for four days. Data were analyzed using completely
randomized design with three treatments and six replicates. Results of this study showed that mean
volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS,
LS, and spermatozoa abnormal of PE goat fresh semen were 1.05 ml, cream, thick,
6.9, ++ – +++, 3,756.67 million cell/ml, 76.67%, 85.83%, and 4.5%,
respectively. Tris, sodium citrate, and
lactose extenders have same ability in maintaining viability of EC goat
spermatozoa during preserved at 3–5oC.
At day-4 storage, percentages of MS and LS for Tris (44.17 and 57.83%)
was no difference significant with sodium citrate (43.33 and 56.67%), and lactose
(43.33 and 54.17%). In conclusion,
Tris, sodium citrate, and lactose extenders have same ability in maintaining
viability of EC goat spermatozoa during preserved at 3–5oC. Tris, sodium citrate, and lactose extenders
could be maintaining 40% in percentage of motile spermatozoa for three days
after preserved at 3–5oC, and suitable for use in artificial insemination (AI)
program
Keywords: Tris, sodium
citrate, lactose, spermatozoa viability, PE goat
Penulis: Muhammad Rizal,
Muhammad Thahir
Kode Jurnal: jppeternakandd160365