Characterizing the Three Different Alginate Type of Sargassum siliquosum
Abstract: This research was
aimed to identify the brown seaweed, to characterize the acid, sodium and
calcium alginate, and to examine the alginate yield. The identification was
done phaenotypically. The extraction method was pretreated by ethanol
depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2 and presipitated with absolute ethanol. The
characterization of alginate was done by FT-IR spectroscopy and Thin Layer
Chromatography by comparing the samples with standard alginate (Sigma, USA).
The key of identification showed that
the species was Sargassum siliquosum. There are similarities in signal
vibration and TLC spots among the samples and the standard. The TLC test was
also showed that those alginates contain mannuronic and guluronic acid. The
highest yield was produced by Sodium alginate (40,34% + 0,21), followed by Acid
alginate (11,51% + 0,15) and Calcium alginate (4,8% + 0,09).
Keywords: alginate,
characterization, Sargassum siliquosum, yield
Author: Ervia Yudiati, Alim
Isnansetyo
Journal Code: jpperikanangg170045