AMMONIATION OF RICE STRAW AND SUPPLEMENTATION OF Paraserianthes falcataria AND Sapindus rarak ON IN VITRO RUMEN FERMENTATION AND METHANE PRODUCTION
ABSTRACT: This study aimed to
observe the effects of rice straw ammoniation and supplementation of Paraserianthes
falcataria and Sapindus rarak on rumen fermentation and methane production in
vitro.Rice straw was ammoniated by adding 2% urea. Rice straw, ammoniated rice
straw, P. falcataria leaves and S. rarak fruits were oven-dried and finely
ground. Experimental treatments were arranged as follow: rice straw (T1),
ammoniated rice straw (T2), T2 80% + P. falcataria 20% (T3), T2 60% + P.
falcataria 40% (T4), T2 80% + P. falcataria 10% + S. rarak 10% (T5), and T2 60%
+ P. falcataria 20% + S. rarak 20% (T6). An amount of 1 g sample from each
treatment was added with 100 ml rumen fluid and buffer mixture (1:2 v/v), and
incubated in a water bath at 39 ºC for 48 h. The incubation was performed in
four replicates and each replicate was represented by four incubation bottles.
Results showed that urea treatment increased gas production of rice straw at 24
and 48 h, higher ammonia production, higher IVDMD, and lower methane production
as compared to the untreated rice straw (P<0.05). Addition of P. falcataria
or S. rarak at lower level produced similar ammonia concentration as ammoniated
rice straw whereas their addition at higher level decreased ammonia
concentration (P<0.05). Paraserianthes falcataria addition to ammoniated
rice straw decreased protozoa population (P<0.05) and S. rarak further
decreased the faunapopulation (P<0.05) as well. Inclusion of S. rarak at 20%
DM (T6) produced the lowest methaneproduction both at 24 and 48 h after
incubation and lowest methane production per unit of DM degraded. It was
concluded that ammoniation of rice straw improved its nutritional quality with
lower methane production, and its mixture with P. falcataria and S. rarak
further increased the quality and lower the production of methane,
respectively.
Key words: Ammoniation, In
vitro rumen, Paraserianthes falcataria, Sapindus rarak, Rice straw
Penulis: Anuraga Jayanegara
Kode Jurnal: jppeternakandd170210
