SPONGE MOLECULAR SCREENING FOR ANTIMICROBIAL GENES BY PCR
Abstract: Molecular
biotechnology approach has been applied on sponge for preventing diseases on
fishery culture. This is important for anticipating and avoiding the using of
amount of sponge in nature. The present study aims to screen the antimicrobial
(oxytetracycline and chloramphenicol) genes of sponge. DNA extraction of
samples was done using the DNeasy Plant mini kit, Phenol-Chloroform and
modification of Phenol-Chloroform methods. The presence of oxytetracycline and
chloramphenicol genes in sponge was detected using Polymerase chain reaction
(PCR) technique. Result of the study showed that four species (Sylotella
aurantium, Acanthella kletra, Gelliodes fibulatus and Auletta sp. were
amplified for oxytetracycline and two species (Auletta sp. and Pericharax sp.)
of sponge were amplified for chloramphenicol at each 226 bp.
Keywords: sponge;
chloramphenicol; oxytetracycl ine
Author: Rosmiati, Emma
Suryati, Andi Parenrengi, Sulaeman
Journal Code: jpperikanangg070021
