MICROBIAL COMMUNITY OF BLACK BAND DISEASE ON INFECTION, HEALTHY, AND DEAD PART OF SCLERACTINIAN Montipora sp. COLONY AT SERIBU ISLANDS, INDONESIA
Abstract: It is crucial to
understand the microbial community associated with the host when attempting to
discern the pathogen responsible for disease outbreaks in scleractinian corals.
This study determines changes in the bacterial community associated with
Montipora sp. in response to black band disease in Indonesian waters. Healthy,
diseased, and dead Montipora sp. (n = 3 for each sample type per location) were
collected from three different locations (Pari Island, Pramuka Island, and
Peteloran Island). DGGE (Denaturing Gradient Gel Electrophoresis) was carried
out to identify the bacterial community associated with each sample type and
histological analysis was conducted to identify pathogens associated with
specific tissues. Various Desulfovibrio species were found as novelty to be
associated with infection samples, including Desulfovibrio desulfuricans,
Desulfovibrio magneticus, and Desulfovibrio gigas, Bacillus benzoevorans,
Bacillus farraginis in genus which previously associated with pathogenicity in
corals. Various bacterial species associated with uninfected corals were lost
in diseased and dead samples. Unlike healthy samples, coral tissues such as the
epidermis, endodermis, zooxanthellae were not present on dead samples under
histological observation. Liberated zooxanthellae and cyanobacteria were found
in black band diseased Montipora sp. samples.
Keywords: bacteria; black band
disease; Montipora sp.; Seribu Islands
Author: Ofri Johan, Dietriech
Geoffrey Bengen, Neviaty Putri Zamani, Suharsono, David Smith, Angela Mariana
Lusiastuti, Michael Sweet
Journal Code: jpperikanangg140054
